Abstract
Summary
A method is described for the establishment of diploid fibroblast cell cultures from human foreskins. Foreskins are minced and digested for a relatively long period of time in standard tissue culture medium containing trypsin and antibiotics. Undigested tissue is removed by filtration, and the cells are isolated by centrifugation and dispensed in culture vessels. This procedure circumvents the explanation of large amounts of minced tissue fragments and yields larger numbers of cells in a short period of time.
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