Binding Sites of Bromoacetylcholine in the Rat Diaphragm 1

Abstract

Summary

To explore the possibility of using bromoacetylcholine as a tagging agent for the isolation of cholinergic receptors, the binding sites of this compound in the rat diaphragm were studied in the presence and absence of bromoacetate. The solubilized membrane fraction of rat diaphragm was found to contain 18 nmole of ¹⁴C-BrACh-binding sites per gram of tissue. The presence of bromoacetate did not change the binding of ¹⁴C-BrACh. The estimated binding sites of ¹⁴C-BrACh were 1000-fold higher than those of α-bungarotoxin binding sites.