Abstract
Summary
G3PDH was isolated from rainbow trout acclimated to 15 and 5°. Anti-sera to each of the enzymes were prepared in rabbits. Microprecipitin tests showed that the short-term (ST) antiserum reacted only with its specific antigen. However, with the long-term (LT) antisera, there was some cross reaction. Ouchterlony double diffusion confirmed these results. Kinetic studies of each of the G3PDH enzymes in the presence of its specific antiserum were carried out. Specific ST and LT antisera significantly inhibited the activity of the enzyme. No significant effect was seen with the antisera to the noncomplementary enzyme. Some cross reaction was seen with the LT antisera in both instances. The Lineweaver-Burke analyses demonstrated no change in the Km while the V max was reduced, indicative of noncompetitive inhibition by the specific antisera.
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