Abstract
Summary
A simple and highly reproducible method was developed for the dissociation of ovine luteal tissue into isolated cell suspensions using a crude collagenase preparation in Medium 199. The procedure required less than 3 hr. The mean yield of cells was 81.8 ± 9.1 × 106 cells/g tissue. The resulting cell preparation synthesized progesterone and responded to the addition of LH or HCG, but not prolactin, with increased progesterone production. The quantity of progesterone produced was related to the level of LH added. The cells were still steroidogenically capable and responsive to LH after storage for 48 hr at 4°.
The PMSG, LH, HCG, and prolactin used were supplied by the National Institutes of Health. The sheep were synchronized with the aid of Sil Estrus implants, a gift of Abbott Laboratories, North Chicago, Illinois. Ovulation was induced with Gonamone, a gift of Fort Dodge Laboratories, Fort Dodge, Iowa.
The authors acknowledge the technical assistance of Bobbie Livingston, Wayne Hopper, and Vaughn Cook.
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