Fetal Lymphoid Tissues: Increase in Antigen-Binding Cells Induced in Vitro by Antigen,LPS,and Aggregated IgG 1

Abstract

Summary

When adult spleen cells were cultured with SRBC, the frequency of specific antigen-binding cells (ABC) either did not change or, at most, increased threefold. The addition of LPS or aggregated IgG to the cultures did not alter this, although the numbers of antibody-secreting cells recovered from the cultures were markedly increased by LPS and decreased by IgG. In contrast, when fetal liver cells were cultured with SRBC and LPS or aggregated IgG, the numbers of SRBC-ABC formed exceeded that found in cultures of adult cells, but there was no change in the small number of PFC produced.