Abstract
Summary
Human T-lymphocytes were enumerated in 10 normal volunteers and 4 pateints by a new method utilizing an automatic smearing instrument to prepare permanent fixed slides of E-rosettes. The results were compared to the commonly employed hemocytometer method.
Results obtained smearing instrument were similar to those obtained by the hemocytometer method with the added advantages of permanent record, convenience of quantitation, and precise morphological identification of rosette-forming cells. As opposed to well-preserved E-rosettes obtained by the automatic smearing instrument, hand-made smears showed disruption of E-rosettes.
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