Abstract
Summary
The component of crude phospholipid responsible for B. matruchotii calcification was isolated. Crude phospholipid, extracted from the microorganism, was separated into five fractions by column chromatography. A single, protein-containing fraction catalyzed apatite formation in a metastable calcium phosphate solution. The nucleating fraction was identified as a proteolipid.
This work was supported by a grant from The Procter & Gamble Company. Amino acid analysis was done by Dr. E. P. Lazzari. Able technical assistance was provided by Mary Anita Stull.
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