Effect of Fasting and Insulin on the Glucagon-lnduced Orthophosphate Incorporation to the Isolated Perfused Rat Liver 1

Abstract

Summary

Isolated perfused fed rat livers spontaneously liberated glucose and ortho-phosphate to the medium; 24-hr fasted rat livers did not exhibit these phenomena. In perfused fed rat livers, glucagon (2 μg) increased glucose output and promoted ortho-phosphate incorporation. In perfused fed rat livers, insulin (250 or 500 mU) inhibited the spontaneous liberation of glucose and orthophosphate. Comparable doses of insulin significantly reduced the glucagon (2 μg)-induced increase in glucose output from perfused fed rat liver, but did not affect orthophosphate uptake by the organ.