Lipoprotein Lipase Activity in the Bovine Corpus Luteum during the Estrous Cycle and Early Pregnancy 1

Abstract

Summary

Lipolytic activity measured at pH 8.6 in bovine corpora lutea exhibited classical properties of lipoprotein lipase (LPL) in terms of serum and heparin stimulation and NaCl inhibition. LPL activity was measured in 23 corpora lutea collected at different stages of the estrous cycle and early pregnancy. The LPL activity in cyclic corpora lutea (μmole FA released/hr/100 mg acetone powder) was low at Days 4-8 of the estrous cycle (3.1 ± 1.5: mean ± SE) and at Days 19-20 (1.6 ± 0.6). However, high activity of the enzyme was found at Days 12-15 of the cycle (11.8 ± 1.8); these concentrations were significantly (P < 0.01) elevated over those found at Days 4-8 and 19-20. The enzyme activity began to decline at Days 16-18 of the estrous cycle (5.1 ± 1.7). Low enzyme activity was found in the corpora lutea removed from two cows at Day 22 of pregnancy.

Progesterone concentrations were measured in 16 of the 23 corpora lutea and a good correlation (r = 0.75, P < 0.01) was found between lipoprotein lipase and progesterone concentrations of the tissue. The data suggest that LPL may be involved in controlling the transfer of fatty acids, including arachidonic, from plasma lipopro-teins to luteal tissue.