Abstract
Summary
The plasma of eight different species was subjected to electrophoresis on polyacrylamide gel, and the position of renin substrate was determined. There are considerable differences in the electrophoretic mobility of the renin substrates tested. Sheep substrate shows the slowest migration and mouse substrate the most rapid. The species tested appear to fall into two groups: slow-moving substrates occurring in the plasmas of sheep, cow, pig, and rabbit and fast moving substrates in man, dog, rat, and mouse. In most species only a single peak of renin substrate appeared, but in man and dog a minor peak was often observed in addition to the prominent major one. The classification of human renin substrate as an alpha-2-globulin is questioned.
The authors thank Drs. H. Goldblatt and E. Haas for the gift of human renin, Dr. F. Katz for antiserum against angiotensin I, and various cooperative persons in Livermore for kindly giving us samples of fresh blood from pigs, cows, sheep, and dogs.
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