Abstract
Summary
The growth characteristics of Japanese encephalitis virus cultivated in Novikoff hepatoma cells grown in shaker culture can be differentially altered by the presence of 6-cis- or cis-9-octadecenoic acid in Swim's 67-G medium. The addition of 125 μ of the 6-isomer per ml medium reduced the number of infectious particles produced, whereas the same amount of the 9-isomer enhanced virus production.
The virus was found to be more stable in cell-free spent medium than in fresh medium. The presence of 125 μ 6-18:1 per ml in fresh medium resulted in a rapid loss of virus infectivity.
This investigation was supported by Pubic Health Service Grant HL 08214 from the Program Projects Branch, Extramural Programs, National Heart and Lung Institute, Grant CA 14003 of the National Cancer Institute and The Hormel Foundation. The authors thank Pat Horvat for her excellent technical assistance.
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