Assay of Kinins by Their Effects on Canine Femoral Blood Flow 1

Abstract

Summary

In pentobarbital anesthetized dogs, close arterial injections of bradykinin and kallidin elicit a dose related increase in femoral blood flow. Treatment with the kininase inhibitor BPP_9α augments the femoral blood flow responses to bradykinin and kallidin by five and threefold respectively. The sensitivity of the preparation permits the detection of 0.5-1 ng of either bradykinin or kallidin in untreated dogs and as little as 0.1 ng of kinin peptides in animals receiving BPP_9α. This sensitivity and the steepness of the dose response curves make this procedure suitable for the assay of kinins.