The Uptake of 14 C-Labeled Hydroxyanthranilic Acid and Enantiomers of Tryptophan,Kynurenine,and Hydroxykynurenine in Human Blood 1

Abstract

Summary

Labeled enantiomers of tryptophan, kynurenine, and hydroxykynurenine were given to five patients with scleroderma. Blood samples were drawn periodically over a 24-hr period. When the d-isomers were given, peak blood levels of radioactivity did not appear until 8 hr, whereas with the natural l-isomers peak levels were reached in 2 hr Considerable radioactivity was found firmly bound to the precipitated plasma proteins from both the l- and d-isomers. Incubation studies confirmed the binding of the d-isomers to the serum proteins. These experiments show that the use of pure labeled l-isomers is required, when studying the metabolism of amino acids in man, and that compounds other than l-tryptophan are bound to serum proteins in significant quantities.

We wish to thank first the patients who volunteered to participate in the study and secondly John Jessep, M.D., for his excellent care of the patients.