Liver Regeneration: An Isolation Perfusion System Employed to Assay Hepatic 3 H-Thymidine Incorporation 1

Abstract

Summary

Isolated normal livers perfused with ³H-thymidine containing suspensions which had been previously circulated through isolated livers either (a) regenerating or (b) “sham” operated, showed equal and relatively low levels of both tissue specific activity and nuclear labeling by autoradi-ography.

When such blood-simulating perfusates, containing ³H-thymidine, are circulated through whole isolated regenerating livers, nuclear uptake is apparent and specific activity is increased > 100 X over levels obtained when the same perfusate is circulated through nonregenerating livers.