Abstract
Summary
The activity of a sarcosine dehydrogenase isolated from a strain of Pseudomonas is enhanced by the addition of Triton X-100, Brij 35, and Tween 80, and is inhibited by deoxycholate and Sar-kosyl NL-97. 2,6-Dichlorophenolindo-phenol, which is used as the oxidant in the dehydrogenase assay, has also been employed as an indicator in the spectropho-tometric determination of the critical micelle concentrations (CMC) of both the nonionic and anionic detergents under conditions optimal for the enzyme analyses. A correlation between the activation or inhibitory activities of the surfactants and their CMC values has been established.
A portion of these studies are from the thesis of J. T. Pinto, submitted in partial fulfillment of the requirements for the Ph.D. degree, The Graduate School of Biomedical Sciences, College of Medicine and Dentistry of New Jersey.
Get full access to this article
View all access options for this article.