Abstract
Summary
With an enzyme degradative technique, isotope inequilibrium of glucose metabolites was demonstrated in intact cells and particlefree supernatants of Ehr-lich ascites tumor using l-14C-glucose as tracer. Inequilibrium was found between glucose and glucose-6-phosphate, glucose and fructose-6-phosphate, glucose and 6-phosphogluconate, while glucoses-phosphate and fructose-6-phosphate were found to be in near-equilibrium within the incubation time investigated. Glucose and lactate were found to be in near equilibrium after 8 min in intact cells. Calculations based on the equilibrium levels found, showed that these inequilibria could not be explained by the effects of the pentose cycle.
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