Abstract
Summary
Hamster embryo cells, following infection with IBR virus, showed malignant transformation. Hamsters of all ages, inbred or random bred, inoculated with two of the transformed cell lines developed solid tumors. Preliminary characterization of the tumors induced by one of the cell lines has indicated undifferentiated sarcomas. Viral specific antigen was detected in about 5 % of the transformed cells and 10% of primary tumor cells in culture. Viral specific antibody was detected in the serum of tumor-bearing hamsters by the indirect immunofluorescent method, but no neutralizing antibodies were found. Infectious virus has not been recovered from either the transformed or tumor cells by cocultivation with bovine embryonic kidney cells.
This investigation was supported by NIH Contract No. DBS-72-2105 from the Food and Drug Administration and USPHS Grant No. AI 08648-05 from the Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.
The authors are grateful to Dr. T. W. Reid, Yale University School of Medicine for the reverse tran-scriptase assay. The excellent technical assistance of Edilea Ellinger and Andrea Tomanik is appreciated.
The HSV-1 transformed hamster cells were obtained from Dr. William Summers of Yale University who had originally obtained them as line 14-012-8-1 from Dr. Fred Rapp of Pennsylvania State University.
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