Abstract
Summary
A double antibody radioim-munoassay has been developed that is capable of assaying and discriminating small amounts of pancreatic amylase even in the presence of amylase isoenzymes. Assays on adult pigs indicate a mean serum concentration of pancreatic amylase of 2.4 μg/ml. To date, no other technique can offer such a discrimination of the pancreatic amylase in the circulation. Following pancreatectomy, the amount of radioimmunoassayable amylase in the circulation disappeared within 48 hr. This was reflected by a simultaneous decline in the serum amylase enzyme activity. Within 48 hr after pancreatectomy, the serum amylase enzyme activity had declined 60%. Thus, in pigs, the pancreas contributes significantly to the circulating amylase levels.
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