Abstract
Summary
The isolated perfused rat liver synthesizes antitrypsin activity in a linear fashion as long as 24 hr. The rate of synthesis may be ten times the rate necessary for physiologic levels in vivo. These data are compatible with the liver as the principal site of synthesis of serum antitryptic activity in the rat. The antitrypsin activity of the rat perfusate resembles that of normal human plasma in lability to heat (56°) and to acid (below pH 6) and in apparent molecular size (elution from Sephadex G-200).
We thank Maria Luz Sevilla for expert technical assistance and Dr. Robert H. Schwartz for antitrypsin typing of the human serum.
This paper is based on work partially performed under contract with the United States Atomic Energy Commission at the University of Rochester Atomic Energy Project, and partially by Grant No. 1-R01-AM110 MET and Contract No. NHLI 71-2221 from the National Institutes of Health, United States Public Health Service. It has been assigned Report No. UR-3490-519.
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