Abstract
Summary
The addition of ADP or 5-HT to stirred suspensions of mouse platelets in plasma incubated at 37° induced reversible aggregation, whereas histamine and catecholamines did not produce this response. Preparations obtained from B. pertussis-injected mice were more sensitive to the aggregative effect of ADP and 5-HT. Enhancement of aggregation induced by ADP or 5-HT occurred in the presence of E or NE but not ISOP. The addition of E and NE before or after 5-HT induced an irreversible type of aggregation while ISOP was ineffective. In the presence of imipramine or phentolamine, aggregation by 5-HT was suppressed; morphine did not seem to influence the response. Further, phentolamine, although inhibiting 5-HT-induced aggregation, allowed platelet shape change in response to 5-HT. Also, phentolamine inhibited the E and NE enhancement of ADP-induced aggregation but did not affect the ADP response per se. Finally, labeled 5-HT appeared to be taken up to the same degree by platelets from normal and B. pertussis-injected mice and was not released following the addition of ADP. These results are compared with those obtained in other species.
The technical assistance of Betty A. Nager and Bobbie Jean Dunathan is gratefully acknowledged.
Get full access to this article
View all access options for this article.