Histamine,Pentagastrin,Methyl Xanthines and Adenyl Cyclase Activity in Acid Secretion

Abstract

Summary

Studies of acid secretion were made in four dogs with a gastric fistula (GF) and Heidenhain pouch (HP) in response to various doses of histamine, pentagastrin, caffeine, aminophylline, histamine plus caffeine, pentagastrin plus caffeine, and aminophylline. Similar but limited studies were made in 16 volunteers using histamine or pentagastrin with or without caffeine. It was found that pentagastrin-stimulated acid output was not increased by the methyl xanthines in dog and man. Acid output in response to histamine was potentiated by caffeine in the dog but not in the human studies. Neither caffeine nor aminophylline when infused alone stimulated acid output in the dog. At the end of the animal experiments studies were made in vitro of adenyl cyclase activity in fundic and antral mucosa. Adenyl cyclase activity in dog gastric mucosa was not stimulated by histamine or pentagastrin.

A preliminary report of this work was published as an abstract in Gastroenterology 60, 649 (1971). These studies were supported in part by College of Medicine (XO43) and Veterans Administration Research Funds as well as Grants Nos. AM 15886 and CA 12191 from the U.S. Public Health Service. Daryl K. Granner is a Veterans Administration Clinical Investigator. The technical assistance of Donna Per-renoud and Carol Butters is gratefully acknowledged. The pentagastrin was supplied by Dr. T. Robitscher, Ayerst Laboratories, NY.