Abstract
Summary
The biosynthesis of the luteinizing hormone releasing hormone (LRH) from3H-tyrosine by rat hypothalamus in vitro has been studied in normal and castrated male rats. After 1 hour of incubation with 3H-Tyr the hypothalamic extracts were chromatographed on Sephadex G-25. Fractions with LRH activity determined by in vivo assays, eluted from this column were passed through a CM-Sephadex C-25 column with a gradient of ammonium acetate 50 mM, pH 5.0–200 mM pH 7.0. Radioactivity which emerged in the fraction with LRH activity was considered as 3H-Tyr incorporated into peptides with LRH activity and this incorporation is higher in castrated than in normal rats.
Get full access to this article
View all access options for this article.