Abstract
Summary
Particles resembling guinea pig leukemia virus were activated from guinea pig nonproducer cells and cultured normal guinea pig cells after chemical treatment. These particles were approximately 100 nm in the mature form, had a density of 1.16-1.17 g/ml, and contained RNA-dependent DNA polymerase activity. The peak of polymerase production was observed between 6 and 8 days after 1-day bromodeoxyruidine (BrdU) treatment. Polymerase activity was reinducible and was always detectable in the BrdU-containing medium of the cultures in prolonged cultivation. The results of screening various chemicals demonstrated that thymidine analogs (BrdU and 5-iododeoxyuridine) were by far the most efficient inducers of guinea pig leukemia-like virus in the cells. Antigenic relationship to known type C viruses has been discussed.
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