Physicochemical Properties of RNA of Salmonid Hematopoietic Necrosis Virus 1 (Oregon Strain)

Abstract

Summary

Infectious hematopoietic necrosis virus (IHNV) (Oregon strain) was propagated in salmonid cell lines and purified by differential and density gradient centrifugation. The virus had a density in sucrose gradients of 1.16 g/cm³. The nucleic acid extracted from purified virus was shown to be RNA as determined by chemical analysis, by its density in a cesium sulfate gradient, and by its sensitivity to ribonuclease. The latter property, together with a nonequimolar ratio of adenylic to uridylic acids, indicate its single stranded nature.