Abstract
Summary
A cloned line of Friend erythroleukemia cells (T-3-Cl-2) can be induced to produce hemoglobin when grown in the presence of 1%-2% DMSO. Such DMSO-treated cells become 30%-60% benzidine-positive in 5-7 days. The control enzyme for hemoglobin synthesis, Δ-aminolevulinic acid (ALA) synthetase becomes elevated after 28 hr in DMSO-treated cells. Furthermore, this synthetase activity increased 2-6 times the level of control cells during the following 3 days. Maximal ALA synthetase activity was found when the culture media contained 2% DMSO. Allylisopropylacetamide alone was found to be as effective an inducer of ALA synthetase as 1% DMSO, and could augment enzyme activity when present with DMSO.
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