Distinction of Serum Inhibitor of Activator-Induced Clot Lysis from α 1 -Antitrypsin

Abstract

Summary

Antiactivator activity of human serum, which inhibits activator-induced clot lysis, was separated from α₁-antitrypsin by affinity chromatography on Sepharose coupled with IgG of antiserum to α₁-antitrypsin. It was found that most of antiplasmin activity formerly found in the antiactivator fraction is actually due to α₁-antitrypsin contaminating the fraction. Antiactivator itself has no or little antiplasmin activity. On the other hand, α₁-antitrypsin possesses antiplasmin activity but has no antiactivator activity.