Feminine Patterns of Androgen Metabolism and Presence of Δ 5,3β-Hydroxysteroid Oxidoreductase in Target Organs of Genetic Rat Male Pseudohermaphrodites

Abstract

Summary

The metabolism of dehydroepiandrosterone-7-³H, androstenedione-1,2-³H, and testosterone-1,2-³H by the preputial glands and genital skin (prepuce) was determined in genetic male pseudohermaphrodites, and in their littermate King-X Holtzman males and females. Both tissues in each of the three animals have an active Δ⁵-3β-hydroxysteroid oxidoreductase system capable of transforming dehydroepiandrosterone to testosterone and testosterone metabolites in a similar fashion to that of the woffian duct and genital tubercle of the fetus of this species as previously reported. The present results also indicate that the pattern of androgen metabolism in both tissues of the rat male pseudohermaphrodites has an elevated amount of 17β-hydroxylated and 3α-hydroxylated 5α-androstane products resembling that of the corresponding tissues of the female and is significantly different from that of its littermate male. Thus, it appears that there is a sex dependent difference in the pattern of androgen metabolizing enzymes and target organs which may be determined at or before birth. The failure to attain a masculine pattern in the male pseudohermaphrodite can be attributed to this animal's inherited deficiency of androgen receptor proteins.