Abstract
Summary
Highly enriched fractions of mature and immature granulocytes were isolated from human bone marrow cell suspensions by Ficoll density centrifugation. Fractions of immature cells contained myelocytes (45%), promyelocytes plus blasts (31%), and only 8% mature neutrophils. Fractions of mature cells contained 85% cells at the metamyelocyte to polymorphonuclear stages of maturation and only 9% immature neutrophils. The cells were well preserved morphologically and are useful for biochemical studies of cell maturation.
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