Abstract
Summary
A virus (RFV) isolated from the urine of a human renal transplant patient and grown in human embryo kidney (HEK) cell culture had properties associated with the polyoma-SV40 subgroup of papovaviruses. Virions with papovavirus capsomeric structure, 37-43 nm in diameter, contained circular, supercoiled DNA of 2.9 × 106 daltons. Density gradient analysis revealed 2 classes of particles in infected cell cultures; full virions at 1.34 g/cm3 and empty virions at 1.29 g/cm3. Both full and empty particles agglutinated human type O erythrocytes. Infectivity and hemagglutinin were resistant to chloroform. Hemagglutination-inhibition tests and plaque neutralization tests in HEK cells indicated that RFV was related to, but distinct from SV40 virus. Antibodies against RFV were present in at least 81% of 400 human adult sera and high titers of HI antibodies against RFV were present in pooled human immune globulin.
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