Abstract
Summary
Poliovirus type I and herpesvirus type I were used as probes to determine whether protein misspecification could account for the finite lifetime (Phase III phenomenon) of cultured normal human diploid fibroblasts (WI-38). Quantity of virus produced, pattern of cytopathology, plaque morphology, and analysis of herpesvirus proteins by polyacrylamide gel electrophoresis did not differ in virus progeny produced in young (Phase II) or old (Phase III) WI-38. These results do not support the notion that misspecification of proteins can account for cell aging in vitro.
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