Survival of Murine Hemopoietic Cells in an in Vivo Culture System

Abstract

Summary

Murine bone marrow cells were labeled in vitro with ¹²⁵IUDR and implanted in diffusion chambers into neutropenic and control mice. Proliferation of granulocytic cells and macrophages in the implanted marrow was not affected by ¹²⁵IUDR. Granulocytic proliferation was enhanced in chambers implanted into neutropenic animals, but the survival of the implanted marrow, judged by the retention of ¹²⁵I within the chambers, was the same in neutropenic and control hosts. These data suggest that the augmented growth in neutropenic hosts was the result of a true increase in proliferation rather than improved survival of the implanted marrow.