Abstract
Summary
The intraventricular injection of 5 μliters containing 30 μg of puromycin, 15 μg of cycloheximide or 10 μg of actinomycin D led to an inhibition of LH release and a fall in plasma LH levels in castrated female rats. Control injections of either saline or an analog of puromycin which lacks its ability to inhibit protein synthesis had little or no effect on the circulating levels of LH.
Since LRF content in the hypothalamus was not significantly lowered by puromycin, the cause of the inhibition of LH release produced by inhibitors of RNA or protein synthesis remains to be determined, but may involve an inhibition of the synthesis of LRF and/or other effects on hypothalamic function.
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