Effect of Dietary Fat on the Release Rate of Cholesterol from Swine Erythrocytes

Abstract

Summary

Three groups of swine were fed for 8 mo the basal ration; the basal ration plus 17% corn oil or the basal ration plus 17% hydrogenated fat which contained 50% trans fatty acids (margarine base stock). The cholesterol content in the plasma was higher in those fed hydrogenated fat diet as compared to those fed the basal or corn oil. However, the cholesterol content in the erythrocytes appeared to be constant regardless of dietary fat. Different dietary fats resulted in considerable differences in the total fatty acid patterns of the plasma and erythrocyte lipids. The release rate of cholesterol from the erythrocytes obtained from swine fed the different dietary fats was compared from cholesterol specific activity changes vs time. The results indicate that the time required to reach 50% equilibration for the erythrocytes and plasma from those fed corn oil, basal diet, and hydrogenated fat was; 1.24, 1.32, and 1.98 hr; respectively. A possible explanation for the slower release rate of cholesterol in those fed hydrogenated fat was given.