Preparation of Peroxisomes from Mouse Liver by Rate-Zonal Centrifugation 1

Abstract

Summary

A procedure is described for the isolation of peroxisomes from mouse liver by two successive rate-zonal centrifugations through sucrose gradients. A feature of the technique is the pretreatment of the animals with Triton WR-1339 and prednisolone to decrease the sedimentability of the lysosomes and to increase that of the mitochondria. On the basis of enzyme activities, preparations can be obtained that contain 40–50% of the peroxisomes of the original liver homogenate, at a purity of over 90%.