Abstract
Summary
Eastern equine encephalitis virus replicated extensively in rabbit kidney cells. Initially, five peptides were detected which are characteristic of cells infected by group A arboviruses. By 5 hr after infection, the two largest nonstructural peptides were no longer detected. Infected cultures labeled from 3-6 hr after infection demonstrated all five peptides. Failure to detect the two largest nonstructural proteins after 5 hr probably resulted from termination of their syntheses rather than increased rates of degradation. This phenomenon may represent a regulatory mechanism governed by viral RNA and/or protein concentrations, since termination was not apparent in cultures pretreated with large doses of interferon.
This work was supported in part by NIH Grant 5ROI-AI-02953, 5R22-AI-02686, and NIH Predoctoral Fellowship 5 GM 41, 441-03. The technical assistance of Alma Tillman, Annie McCoy, Carlie White, and Mary White is gratefully acknowledged.
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