Studies of Cyclic AMP Effect on Release of Unsaturated Vitamin B 12 Binding Capacity from Human Blood Cells 1

Abstract

Summary

Cyclic AMP and other agents capable of elevating cyclic AMP levels were incubated with human blood cells to test ability to inhibit unsaturated vitamin B₁₂ binding protein release. Except for NaF, none of the agents tested showed a significant effect on UBBC increment at 24 hr compared to controls. As shown previously, NaF fully prevented UBBC release, even in the presence of added Li⁺. From these data, it is concluded that release of unsaturated vitamin B₁₂ binding protein from human blood cells is not mediated primarily through changes in intracellular cyclic AMP levels.