Abstract
Summary
Bile-duct ligation causes a 5-10-fold increase in rat-liver alkaline phosphatase activity. The alkaline phosphatase in bile-duct-ligated rat liver is identical to electrophoretic mobility on polyacrylamide gel, rate of sedimentation in a sucrose gradient, rates of heat and urea denaturation, pH optima, and Michaelis constants. Since the “induced” and base-level enzyme are identical by all criteria studied, it is unlikely that the increase in alkaline phosphatase activity is due to activation of a preformed enzyme. Enzyme induction appears a more likely mechanism.
This work was supported by Research Training Grant AM-5424 and Research Grant AM-10571 from the National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, and by a grant from the John A. Hartford Foundation, Inc. Dr. Kaplan is the recipient of Research Career Development Award AM-31760 from the National Institutes of Health.
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