Abstract
Summary
The incorporation of radioactive nucleosides into rabbit granulation tissue RNA has been studied in vitro. Polyvinyl sponge implantation was used to obtain tissue and inflammatory fluid. Decreased nucleoside incorporation occurred in tissue incubated with inflammatory fluid or blood. These fluids contained extracellular enzymes that degraded radioactive uridine and cytidine to uracil which is not incorporated. Since granulation tissue also consists of a large amount of enzyme-containing inflammatory fluid in its extracellular space, quantitative studies of RNA synthesis in this tissue may be affected. Utilization of incubation times of less than 40 min, washing of tissue, and use of a low phosphate concentration in the incubation fluid will minimize these effects. Failure to detect these effects would have led to erroneous interpretation of the incorporation rate data obtained.
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