Initiation Factors in Protein Synthesis of Liver and Skeletal Muscle of Rats Force-Fed a Threonine-Devoid Diet 1

Abstract

Summary

Young rats were force-fed for 3 days a complete purified diet or one devoid of threonine. Initiating factors prepared from the ribosomes of livers of experimental animals stimulated polyphenylalanine synthesis and the binding of ¹⁴C-phe-tRNA and ¹⁴C-met-tRNA to a greater extent than those from the livers of control animals. In contrast, initiation factors and also elongation factors showed decreased activities in the skeletal muscles of experimental animals in comparison to those from control animals.