Desulfation of Sulfated Polyanions by Rat Gastric Mucosa 1

Abstract

Summary

Rat stomach mucosal scrapings contain enzymes which attack chondroitin 4-and 6-sulfates, heparain, sulfated polyanions from gastric and duodenal mucosa and aryl sulfates. Inorganic and organic sulfates were liberated from the sulfated polyanions. Fractionation with (NH₄)₂SO₄ afforded enrichment of the sulfatase and depolymerase enzymes in the precipitate obtained at 60–72% saturation. Enzyme activities were stable for at least two months when stored in pH 4.0 acetate buffer under toluene at 5°.