Abstract
Summary
The kinin-forming potential of a cultured cell line of mouse fibroblasts, L-929, has been studied. The fibroblasts were found to form kinin at acid pH from both rat plasma and Murphy-Sturm lymphosarcoma kininogen substrate preparations. The kinin formed was destroyed following incubation with carboxypeptidase B. The kinetics of kinin formation relative to incubation time and concentration of fibroblasts also were studied. None of the conventional plant, mammalian, or synthetic protease inhibitors had any significant effect on the kinin-forming activity of the fibroblasts. Phenylpyruvic acid, however, did inhibit this activity. The activity appeared localized in the soluble protoplasmic portion of the cell, as determined by ultracentrifugal technique.
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