Abstract
Summary
The isotope method of detection of corrective factor activity for cultured Hurler fibroblasts using radioactive sulfate (35SO4) has been confirmed and improved as a sensitive screening method. Its limitations have been identified and discussed. Using this method it has been shown that cultured Hurler fibroblasts turn over 2–3 times less macromolecular 35SO4-AMPS in 12–24 hr than do cultured normal fibroblasts. The results also establish the presence of a heatlabile corrective factor in fetal calf serum, which, because of its similar, though smaller effect on normal cells, is presumed to be nonspecific.
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