Abstract
Summary
By means of co-chromatographic techniques using 3H- and 14C-labeled amino acids in appropriate reaction mixtures, it is possible to detect and compare tRNAs of herpesvirus type 2 infected with uninfected H. Ep. 2 cellular controls. Individual tRNAs are separated by reversed-phase Freon column chromatography. In terms of picomoles of amino acid accepted per phenylalanyl-tRNA peak, herpes virus type 2 infected preparations are 110 times more active than uninfected controls. In comparative experiments for 9 other amino acids tested there are either no differences or only 3- to 8-fold increases in activity by one system or the other. Possibilities as to the demand for phenylalanine by viral infected preparations are suggested but whether or not a different tRNA is coded by the virus is not known.
Appreciation is expressed to Mrs. Donna Fisher and Mr. Arthur Livermore for excellent technical assistance. The work is supported by Grant E-423B of the American Cancer Society and by funds provided by the American Cancer Society, Oregon Division.
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