Abstract
Summary
The synthetic decapeptide, pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 (Gn-RH) was evaluated for induction of ovulation in the cycling rat. All injections of the decapeptide were made at 9:00 am. At the intravenous dose of 5 μg, Gn-RH induced ovulation only during proestrus in both the normal cycling and pentobarbital-blocked rat. Serum LH elevation following Gn-RH was greater at proestrus than at metestrus or diestrus-1, and least at diestrus-2. Peak serum levels of LH were, respectively 60.6, 33.5, 33.5, and 18.8 ng/ml NIH-LHS16. In the ovulating rat, serum LH elevations after Gn-RH were similar in magnitude and duration to the normal proestrous levels.
The authors wish to thank Dr. G. D. Niswender for his generous gift of LH antisera and Jacquelyn Dausman for her excellent technical help.
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