Effects of Intrauterine Foreign Body on the Localization of [ 3 H-]Estradiol in Rat Uterine Tissues,Polymorphonuclear Neutrophiles and Eosinophiles 1

Abstract

Summary

Effects of unilateral placement of an intrauterine foreign body (IUFB) on the localization of [³H] estradiol were studied in ovariectomized estrogen-primed rats, using dry-autoradiography. Autoradiograms of control and IUFB horns were prepared at 2, 10 and 60 min after [³H]estradiol administration. In the control uterine horn, nuclear concentration of radioactivity was observed in cells of the lumenal and glandular epithelium, substantia propria and muscularis. In the IUFB uterine horn, no nuclear concentration of radioactivity was observed in the cells of the outer layers of the multilayered metaplastic lumenal epithelium, but little radioactivity existed in cells of the basal layer of the lumenal epithelium and the underlying cells of the substantia propria with higher nuclear concentrations in the stromal and glandular cells. Infiltrating polymorphonuclear leukocytes and eosinophiles did not accumulate radioactivity. These observations suggest that an IUFB alters uptake of estrogen in proliferating epithelial cells adjacent to the uterine lumen, and that the infiltrating white blood cells are not target cells for estradiol.