Adipose and Liver Tissue Enzyme Profiles in Obese Hyperglycemic Mice 1

Abstract

Summary

In order to determine metabolic abnormalities associated with obesity in the obese hyperglycemic mouse, enzyme levels in adipose and liver tissue were measured in three groups of mice. One lean group and one obese group were fed ad libitum. The third group consisted of obese mice subjected to weight gain control by dietary restriction and exercise. Levels of adipose tissue enzymes associated with lipogenesis were higher in obese mice than the lean mice. The data also indicated that obob mouse adipose tissue enzymes were not appreciably affected by diet restriction and exercise. However, liver tissue enzymes associated with lipogenesis and gluconeogenesis, which are normally elevated in obese mice, were restored to normal levels when the obese mice were on a weight gain control schedule. These data indicate that some differences in enzyme profiles observed in a genetically obese mouse are secondary adaptations caused by changes in either dietary intake or spontaneous activity.