Abstract
Summary
Pretreatment of L cells with mouse interferon diminishes their responsiveness to interferon induction (blocking effect). The blocking property of interferon preparations is liberated simultaneously with interferon from stimulated cultures. Both activities are reduced to the same degree by partially inhibitory concentrations of actinomycin D. Chromatographic purification of interferon (specific act, 1–3×106 units/mg protein), fails to dissociate a separate blocking component from interferon. Nonpurified and chromatographed interferon preparations display the same blocking potency in relation to interferon content. Blocking can be partially or completely reversed by increasing the multiplicity of infectious NDV. Under the same conditions, reversal of blocking does not occur with excessive inducer concentrations of NDVuv and poly I:C.
The authors are grateful for the competent assistance of Marilyn J. Mundy.
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