Oxidation of 14 C–Glycerol Administered by Ventriculocisternal Perfusion in the Dog 1

Abstract

Summary

1–3¹⁴C–Glycerol in artificial CSF was perfused from the third ventricle to the cisterna magna in dogs. The appearance of ¹⁴CO₂ in the perfusate and in blood from the confluens sinuum was studied after 95, 135, and 175 min of perfusion. Specific activity of CO₂ in the perfusate at all times exceeded that of the venous blood which in turn exceeded that of arterial blood. The calculated rate of oxidation of glycerol was between 2 and 10 nmoles/min in the majority of animals. The disappearance of counts from the perfusate indicated a consistent uptake of the labeled glycerol. A significant fraction of the labeled glycerol was recovered in the perfused tissue.