Abstract
Summary
A simple method is described for the preparation of primary and secondary monolayer cultures of neurons from embryonic mouse brain and cord tissues. Key points of technic were the use of culture dishes containing a thin layer of serum to facilitate cell adherence and a medium selective for neurons that contained 600 mg% of glucose and 0.06% Tween 80. Evidence is presented to show that neurons underwent limited cell division in vitro. Primary and secondary cultures survived 10 to 12 days. Cultures could be transferred 2 to 6 times.
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