Abstract
Summary
Chinese hamster fibroblasts derived from seminiferous tubules grow well in Eagle's minimal essential medium containing glucose and 10% fetal calf serum. This medium contains no vitamin E; nevertheless, addition of the vitamin has no effect on the growth of these cells. When pyruvate is substituted for glucose as a carbon source, there is a great decrease in the growth rate which can be alleviated by d-α-tocopherol. The growth rate with pyruvate is much less than when no added carbon source is used.
This work was supported by U.S. Public Health Service Grant AM 14508 from the National Institute of Arthritis and Metabolic Diseases.
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